Protective Effect of Celery ( Apium graveolens L. ) Essential Oil on the Experimental Model of Cuprizone-induced Multiple Sclerosis in Male C57BL/6 Mice

Considering the beneficial effects of Apium graveolens L. (Celery) on the nervous system, this study elucidates the protective effect of CEO on the experimental model of cuprizone-induced MS in male C57BL/6 mice. Frothy mice were allocated into four experimental groups: control, cuprizone (chew pellet containing 0.2 %CPZ), CEO (800 mg/kg), and CPZ+CEO. Animals received treatments based on their groups for 5 weeks. Finally, reflexive motor behavior and serum antioxidant levels were determined. Based on the findings, ambulation score, hind-limb suspension, front limb suspension, and grip strength significantly decreed in the mice treated with CPZ (p < 0.05). Hind limb foot angle, surface rights, and negative geotaxis significantly increased in the animals treated with CPZ (p < 0.05). Co-administration of CPZ+CEO significantly reduced the adverse effects of CPZ on ambulation score, surface righting, hind limb suspension, grip strength, and negative geotaxis (p < 0.05). Co-administration of CPZ+CEO significantly diminished the adverse effects of CPZ on the number of crosses in the open field test and duration on the rotarod (p < 0.05). Serum MDA activity increased while GPx, SOD, and TAS decreased in the mice treated with CPZ (p < 0.05). Co-administration of CPZ+CEO significantly reduced the adverse effects of CPZ on serum antioxidant levels (p < 0.05). These results suggested the protective effect of CEO against CPZ-induced MS mediated by its antioxidant activity.