The effect of a mixture of native Iranian probiotics (Lactobacillus rhamnosus, Lactobacillus casei and Lactobacillus holoticus) on liver tissue changes in male rats exposed to cadmium chloride

Probiotics are beneficial organisms that can be used as a suitable tool against absorption and possible poisoning by heavy metals. The aim of this study was to investigate the effect of a mixture of native Iranian probiotics (Lactobacillus rhamnosus, Lactobacillus casei and Lactobacillus holoticus) on liver tissue changes in male rats infected with cadmium chloride. 21 adult male Wistar rats were divided into 3 groups of 7: control, cadmium chloride, cadmium chloride with a mixture of native Iranian probiotics. Cadmium chloride (3 mg/kg) was injected intraperitoneal and by probiotic gavage (109 CFU/ml) for 30 days. At the end of the test, blood samples were taken from the heart to measure the serum level of liver enzymes, liver tissue was extracted and histological studies of the liver were performed after hematoxylin-eosin staining. Data were compared between groups based on one-way ANOVA and Tukey test. Serum levels of liver enzymes in the group receiving cadmium chloride showed a significant increase compared to the control group and in the group treated with a mixture of native Iranian probiotics showed a significant decrease. Also, morphological changes of liver tissue in the group treated with probiotics showed a decrease in tissue damage compared to the cadmium chloride group. According to the findings of this study, the use of a mixture of native Iranian probiotics (Lactobacillus rhamnosus, Lactobacillus casei and Lactobacillus holoticus) can improve the induced damage of cadmium chloride in liver tissue.