The Effect of 2-Day Intermittent Hypoxia-Hyperoxic Training on Anaerobic Metabolism

Researchers have studied practical applications of high-intensity interval training and hypoxic training. PGC-1α, IGF-I, and HIF-1α are generated from high-intensity interval training and affect muscle cells. Altitude training also produces HIF-1α, which induces erythropoietin and increases the number of red blood cells. However, due to the limit on training intensity and cycle, it was replaced by normobaric hypoxia training. To investigate the effects of 2-day high-intensity interval training on muscular anaerobic metabolism at varying oxygen concentrations for 2 days. Ten subjects performed 2 consecutive days of intermittent hypoxia-hyperoxic training (IHHT); intensity of training was determined by the sprint test results. IHHT comprised 4 sets of 3 repetitions for 40 seconds (4 × 3 × 40 s); the intensity and oxygen concentration were 80% of maximal effort and 13% oxygen for the first and third sets, and were 90% of maximal effort and 21% oxygen for the second and fourth set; participants walked for 3 min wearing an oxygen mask (O2 80%) before each set. Differences in biological parameters between the two days of training was analyzed by t-test. The difference of mean lactate levels between two days were 0.96 ± 0.75 mmol/l at Set 3 (P = .003) and 1.05 ± 1.12 mmol/l at Set 4 (P = .016), respectively. Mean ammonia concentrations at Set 4 were 117.70 ± 29.8 μmol/l for the first day and 94.50 ± 14.45 μmol/l for the second day (P = .057). The difference of heart rate were 4.20 ± 5.05 min-1 at Set 1 (P = .027) and 4.00 ± 5.48 min-1 at Set 2 (P = .046). Two-day intermittent hypoxia-hyperoxic training affected lactate and heart rate.