Evaluation of the relationship between ABCB11 gene polymorphism and the incidence of gallstones and related SGOT and SGPT biochemical factors in the Iranian population

Gallstones are one of the most common gastrointestinal diseases because genetic factors have not been yet identified. The ABCB11 gene locates on chromosome 2 and generates the Bile Salt Output Pump (BSEP) in human encoding by creating gallstone. Our aim of in the present study was to investigate the relationship between liver biochemical factors and ABCB11 gene polymorphism with the risk of gallstone.

The study was performed recruiting 100 gallstone patients and 100 healthy individuals in Golsar Hospital, Rasht, in 1397. After sonography, subjects were divided into patients and healthy groups and the relationship between disease and ABCB11 gene polymorphism was examined by ARMS-PCR. Typically, 10 samples were sampled for the required sequencing results. Differences between the two groups were determined by statistical analysis.

In the present SGOT study, SGPT level was elevated in patients in comparison with healthy individuals (P <0.05). The frequency of ABCB11 gene C allele between patient and healthy was 22.5% and 22% of the budget, respectively. There is no significant relationship between the frequency of alleles and genotypes in patients compared to healthy cases (P ˃ 0.05). Under these conditions, the frequency of TT, TC and CC genotypes among individuals over 60 years of age were 27, 21 and 3, respectively. There is no significant relationship between gender and age with disease (P /0 0.05). The mean age of patients over 60 years (P = 0.0) was significantly higher than healthy. People with homozygous TT genotype were more likely to develop the disease.

The increased level of biochemical factors can predicts possibility of liver damage. The lack of correlation between ABCB11 and disease may be due to genetic differences between different races. Further studies with a larger group is essential.