Investigating on antioxidant activity of zebrafish embryo extract (Danio rerio)

Zebrafish is considered as a useful laboratory model due to its diverse characteristics, including self-renewal. The embryo of this fish has unique characteristics in the early stages of development, and its effects were observed in various studies. The differentiating factors present in stem cells isolated from zebrafish embryos are effective in improving the functional status of patients, and exposure to zebrafish embryo extracts in the early stages of development may increase the expression of multipotent stem cells and exert positive effects.  In this study, we investigated the antioxidant properties of the zebrafish embryo extract in different embryonic stages of development.The Zebrafish egg extract was prepared in different embryonic stages. Its effect in concentrations of 0.5, 1, 1.5 and 2 mg/ml on DPPH free radical scavenging activity, ABTS radical inhibitory activity and iron reducing power (FRAP) were investigated. The studied groups included protein extracts in morula, blastula and gastrula stages. According to the obtained results, the amount of protein varied in different embryonic stages and the amount of protein increased with the progress of fetal growth and the amount of fat decreased.The protein extract in the gastrula stage showed the highest level of DPPH inhibition and iron ion reduction at a concentration of 2 mg/ml compared to the morula and blastula groups (P<0.05). Also, the protein extract in the embryonic stage of blastula had the highest inhibition of ABTS at a concentration of 2 mg/ml compared to other groups (P<0.05).