The effect of simulating staying at moderate altitude with thyme consumption on cardiomyopathy and liver enzymes in Rats with type 2 diabetes

The aim of study was to investigate the effect of simulating staying at medium altitude along with consumption of thyme on the cardiomyopathy rate of type 2 diabetic rats and their simultaneous effect on liver enzymes.

For this purpose, 40 three-month-old rats (initial weight 220 ± 20 grams) were divided into five groups of eight: 1- healthy control (NC), 2- diabetic (D), 3- diabetic + thyme (D + Th), 4- diabetic + hypoxia (D + H) 5-diabetes + hypoxia + thyme (D+H+Th) were divided. In order to induce type 2 diabetes, streptozotocin was injected intraperitoneally at a dose of 37 mg/kg. The moderate altitude group was alternately placed for eight weeks in night sleep conditions at a simulated altitude in a hypoxia chamber, and thyme group was supplemented with thyme in the form of 400 mg/kg of hydroalcoholic extract in 30 ml of drinking water. Cardiomyopathy was measured through two indices of apoptosis (western blot) and fibrosis (staining) of the myocardium, and diabetic indices, liver enzymes using methods Calorimetry was measured by biochemistry company.

The results showed that 8 weeks altitude, thyme supplement and the combination reduce the fasting glucose level in diabetics (P<0.05). Also, the results of the multivariate analysis of variance test showed Apoptosis and fibrosis showed a significant decrease in myocardial type 2 diabetes with exposure to high altitude and simultaneous consumption of thyme (p<0.025). Also, moderate altitude and consumption of thyme extract had no significant effect on the serum levels of ALT and AST transaminases.

Simulated moderate altitude and consumption of thyme in this research decreased fasting blood sugar and on the other hand, by reducing apoptosis and fibrosis, it prevented cardiomyopathy in the heart of type 2 diabetic rats, but these two variables did not cause a significant decrease in ALT and AST enzymes.