Partial Proteomics Analysis of Montivipera raddei Venom

The snake venom is a potent source of a variety of drugs and therapeutic components. This study aimed to isolate and characterize some of proteins in Montivipera raddei venom. The protein bands and spots obtained by SDS-PAGE and two-dimensional electrophoresis were analyzed. The separated protein spots based on isoelectric point and molecular weight were scattered in the 15 to 66 kDa ranges and pI from 5 to 8. Six proteins was more extensively characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/TOF) analysis. These characterized proteins included Zinc metalloproteinase-disintegrin-like ecarin, L-amino-acid oxidase, metalloproteinase kistomin, Thiamine-monophosphate kinase, Ancrod, Acidic phospholipase A2.