Investigation of effect of oxaliplatin and paclitaxel drugs on colon cancer cells(HT29) and analysis of apoptotic genes expression caspase 3, caspase 9 and P53

In this study the effects of paclitaxel and oxaliplatin on colon cancer cell line (HT29) and expression of apoptotic genes caspase 3, 9, and p53 were examined. Using the MTT method, the cytotoxicity of paclitaxel and oxaliplatin and synergic effect at different concentrations on the HT29 cell line was assessed. The IC50 of paclitaxel and oxaliplatin was determined.The expression level of caspase 3 and 9 was assessed using the Real-Time PCR method after the cells had been exposed to the IC50 concentration. Apoptosis of the cells was done using Annexin V/PI and DAPI staining. The treatment of cells revealed that paclitaxel and oxaliplatin at concentrations of 3.25 and 0.00062 µg/ml , respectively, have the most cytotoxic effects and its IC50 value was determined to be 12.5 µg/ml and 0.00016 and treatment groups at concentrations of 3.25 and 0.00062 µg/ml , respectively, decreased the cell viability and its IC50 value was determined to be 12.5 µg/ml and0.00016µg/m. The expression level of caspase 3 and 9 P53 was also increased in the treated colon cancer cell line. DAPI staining showed apoptosis in the simultaneous treatment groups with. Using Annexin V/PI reveals that 98 percent of the cells in the control group are healthy and a considerable number of the cells in the treated groups have undergone apoptosis. Is. It is clear that paclitaxel and oxaliplatin are effective options for treating colon cancer given their cytotoxicity and stimulation of the apoptotic process in colon cancer cell lines.