Effects of selenium nanoparticles on the performance of antioxidant enzymes and ions of rainbow trout eggs (Oncorhynchus mykiss)

In this research, the effect of selenium nanoparticles on the performance of antioxidant enzymes, including superoxide dismutar (SOD), glutathione peroxidase (GPx), malondialdehyde (MDA), and catalase (CAT) levels, and male reproductive egg ions of rainbow trout was investigated. The number of 144 fertile pieces of 4-year-old Iranian females was divided into four treatments in three replications. The treatments were fed with diet containing zero (control), 0.5, 1 and 2 mg of selenium nanoparticles per kg of diet for 60 days until egg maturation. Based on the results of the research, the values of SOD, CAT, and MDA have shown significant differences in the eggs from different treatments (p<0.05). The lowest level of SOD enzyme and the highest level of CAT enzyme were observed in the control treatment, which showed a significant difference with other treatments. There was no significant difference in the amount of GPx enzyme between different treatments (p>05). The amount of MDA enzyme showed a significant difference among the treatments, while the control group had the highest level of 363±16.8 mmol/ml and the fish fed with selenium nanoparticles at 2 mg/kg had the lowest level of 181±9.5 mmol/ml Comparing the average amounts of magnesium and calcium among different treatments showed a significant increase in the amounts of the mentioned ions in the treatment fed with selenium nanoparticles at the rate of 2 mg/kg of food compared to other treatments (p<0.05). In general, considering the decreasing role of antioxidant enzymes and its increasing role in the amounts of magnesium and calcium inorganic compounds in the eggs of mothers fed with more selenium nanoparticles, this essential micronutrient can be used as It should be considered as a suitable candidate for further research in order to improve the quality of spawning eggs of female rainbow trout by adding to the diet.