Effect of Percolation and Microwave-assisted Extractions on Bioactive Compounds of Red Alga Gracilariacorticata

This study aimed to apply percolation and microwave-assisted extraction methods to extract phenolic, carotenoid and chlorophyll compounds from Gracilariacorticata red algae. During this study, in the MAE method the solvent type (water, ethanol, and methanol), time (10, 20 and 30 min), power (90, 180 and 270 W) and solvent-to-sample ratio (5, 12.5 and 20) were used as variables. Also, in the percolation method, the variables were solvent type (water, ethanol, and methanol), time (24, 48 and 72 h) and solvent-to-sample ratio (5, 12.5 and 20). Based on the results obtained during this study, it was found that the percolation method the solvent and time interaction, solvent and solvent to sample ratio interaction had a significant effect on the chlorophyll a content (p<0.05). To the extract carotenoids in the percolation method, the most effective parameters were type 1 solvent (water) and solvent to sample ratio of 20. The variables of percolation and MAE methods had no significant effect on phenol content (p>0.05). In the MAE extraction method, the solvent-to-sample ratio and the square solvent type had a significant effect (p<0.05) on the chlorophyll content. For extraction of carotenoids using the MAE method, solvent type, and solvent to sample ratio had a significant effect on extraction efficiency (p<0.05).