Assess Effect of Nitrogen Fertilizer and Phosphorous Biofertilizers (Azospirillum and Pseudomonas) on Barley Crop Production and Seed Protein Content

Azospirillum is effective in biostabilization of nitrogen and Pseudomonas is effective in dissolving soil phosphorus.
Current study was conducted to assess effect of different level of nitrogen and biofertilizer on seed yield, its components and protein content.
This research was carried out via spilt plot factorial experiment based on randomized complete blocks design with three replications along 2014-2015 year. The main factor included nitrogen (N0%=0 or control, N50%=75, N75%=112.5, N100%=150 kg.ha-1). The sub factor at first consisted Azospirillum (Azo0: non use of Azospirillum or control, Azo1: use of Azospirillum) and then Pseudomonas (Pse0: non use of Pseudomonas or control, Pse1: use of Pseudomonas).
The results showed that the application of nitrogen fertilizer had a significant effect on seed protein content, seed protein yield, plant height and seed yield at 1% probability level, also inoculation of Azospirillum increased the effect of nitrogen fertilizer on these traits. Mean comparison result of different level of Pseudomonas indicated that maximum seed yield (4800 kg.ha-1) was noted for Pse1 and minimum of that belonged to control treatment. So Pseudomonas inoculation increased seed yield by 8% compared to the control treatment (no bacterial inoculation). As for Duncan classification made with respect to interaction effect N × Azo the maximum and minimum amount of seed yield belonged to N100% and Azo1 (5710.9 kg.ha-1) and control (3981.3 kg.ha-1). Inoculation of the plant with Azospirillum increased the seed protein content by 14.8%. Also inoculation of Pseudomonas caused the highest amount of seed protein content of 13.56 (g.100 g-1).
Inoculation of Pseudomonas fluorescence also had a significant effect on all the mentioned traits. Inoculation of Azospirillum and 75 kg N ha-1 significantly increased seed yield and the use of Azospirillum led to save 25% of the nitrogen fertilizer and can be advised to producers.