Effect of culture supernatant of three probiotic bacterial strains of Bifidobacterium on Pseudomonas aeruginosa

Probiotics are living microorganisms that, if prescribed in sufficient quantity, have beneficial effects on the host's health. Consuming a large number of live bacteria increases the possibility of infection and inflammatory reactions, especially in people with a weak immune system. Therefore, in recent years, new terms such as postbiotics have been added to the literature of researchers. This study aimed to investigate the antimicrobial effect of the culture supernatant of three probiotic strains of Bifidobacterium as a postbiotic on the growth of Pseudomonas aeruginosa bacteria in laboratory conditions.

In order to conduct this experimental study, three probiotic strains of Bifidobacterium bifidum, Bifidobacterium longum, and Bifidobacterium lactis as well as Pseudomonas aeruginosa, were cultivated. Based on the standards, the lack of contamination of probiotic culture was checked and finally, after preparing the culture supernatant of probiotics, the effect of each probiotic strain on inhibiting the growth of Pseudomonas bacteria was compared to the negative and positive control.

  The greatest inhibition of growth was achieved by Bifidobacterium bifidum. (16/33±1.52mm). No significant difference was observed between groups Bifidobacterium bifidum and positive control. The synergistic effect between probiotics was not seen.

Probiotics and postbiotics can act as natural antibiotics against harmful pathogens. For appropriate and targeted exploitation, it seems necessary to identify a strain with a better effect on each pathogen.