Comparative evaluation of methicillin-resistant Staphylococcus aureus infection and carriers of Helicobacter pylori in restaurant staff

Staphylococcus aureus is the most important human pathogen. Staphylococcus aureus is considered as a coagulase-producing strain and has extensive enzymatic and toxin activity. The nostrils and perineum are the major centers of Staphylococcus aureus and Staphylococcus epidermis. Helicobacter pylori is the most common human infectious disease, causing gastric infections in more than 50% of people worldwide. The aim of this study was to compare the incidence of methicillin-resistant Staphylococcus aureus and Helicobacter pylori carriers in restaurant staff.

The present study is a descriptive cross-sectional study that was taken from the nasal swab and the palm of the hand to take samples and the food preparation department (restaurant kitchen) as well as the feces of 54 employees located in the active restaurant in Tehran with Gathering age, sex, history of gastrointestinal disease and previous occupation were collected. The culture medium used was Müller Hinton Agar (Merck-Germany) which was prepared according to the instructions and the discs purchased from Hi media (Himedia India) were quality controlled. DNA extraction from fecal samples was performed using Sinagen commercial kit according to the instructions provided by the kit and Multiplex PCR test was used to identify vacA, cagE, cagT, cagA and hrgA genes as well as BlaCTX-M, 16SrRNA and mecA.         

In this study, the results of antibiogram from 54 cases of coagulase positive and negative staphylococcal infections and Helicobacter pylori infection were evaluated. Percentage of resistance of each of the different isolates to antibiotics used as gentamicin 11%, amikacin 21%, oxacillin 48%, penicillin 67%, ciprofloxacin 19%, erythromycin 49%, methicillin 79%, and tetracycline 24%. Percentage of resistance of different isolates was seen.

Considering the high prevalence of Helicobacter pylori in people working with clinical signs of enteritis, gastric infection, it can be stated that Helicobacter pylori has an important role in causing enteritis and as a carcinogen in the studied samples. Due to the presence of bacteria that can be transmitted to others, it is necessary to identify and treat human carriers.