The effect of Sargassum ilicifolium extract on TGFβ1 and VEGFA gene expression during on skin linear wound healing in rats

Sargassum ilicifolium is a multicellular brown algae that contains beta - carotene and fucoxanthin pigments, soluble acids of immune stimulants such as phycocyanin, polysaccharide, iron, zinc and essential amino acids, which promote safety. In this study, the effect of Sargassum iliisifolium algae extract on linear wound healing percentage and its effect on TGF β 1 and VEGFA gene expression in rats were investigated.

This research is an experimental study that was performed in the laboratory of Parand University of Medical Sciences. For this study, 30 male Wistar rats weighing 180 gr were used and 2 cm deep linear dermis was created from the neck to the waist of rats. Mice were divided into 3 groups of ten: The control group,The group received alpha ointment as a positive control drug and The group algae extract of Sargassum iliisifolium. The groups were examined for both wound size and expression of VEGFA and TGFB1 genes .

The alpha group had a 10.38 fold increase in TGFΒ1 gene expression compared to the control group, with a probability value of P <0.001. Compared with the algal group and the control group, it had a 5.39 increase in gene expression with a probability equal to P <0.001 . Also in assessing VGEFA gene expression :the alpha group significantly increased from the control group by 1.86 times the increase in gene expression with a probability value of p <0.044 , the sargassum algae group compared to the control group by 1.70 There is significant difference between the increase in expression and the probability value of p <0.040 . In terms of the percentage of wound healing, it shows that: the control group, reduced the wound length from 2 cm to 0.64 cm, the alpha group reduced the wound length from 2 cm to 0.5 cm and sargassum algae, the amount of wound has been reduced from 2 cm to 0.3 cm

Sargassum iliisifolium algae extract due to its antibacterial, anti -inflammatory, antioxidant and antimicrobial properties and due to the presence of alginate in it biometrically heals wounds. Available in the market, however, sargassum algae extract make a significant difference in increasing the expression of TGFβ1 and VEFGA gene .sargasum algae extract may use as a wound healer to heal skin damages