Evaluation of Exosomes Extracted from AGS Cells Treated with Nano Coumarin Effect on the Induction of the External Apoptotic Pathway in Drug-Resistant Gastric Cancer Cells

 Gastric cancer is one of the most common gastrointestinal cancers in the world, Iran and Ardabil. The used of exosomes for drug packaging to inhibition of drug-resistant of cancer cells is a new method. This study aimed to evaluate of extraction exosome of the AGS cell line to inhibit of MKN45 cell line.

 Exosomes in concentration LC50 and LC75 nano coumarins were extracted from supernatants of cultured AGS cells using Exosome Isolation Kit and confirmed by DLS, and their effect was applied to drug-resistant MKN45 cells. Inhibition test (MTT) and expression of Caspase 8, Caspase 9, BCL2, Bax, and P53 genes were measured.

 The results showed that the extraction of exosome with concentration LC75 From AGS on MKN45 in addition to the concentration-dependent inhibitory role of impact on a significant change in gene expression Caspase 8, BCL,2, and P53.

 Increased expression of genes Caspase 8, BCL2, and P53 in exosome treatment at LC75 level indicate induction of apoptosis from the external pathway by exosomes in resistant cells, as evidenced by the decrease in the expression of miR-21 oncomir and the increase in miR-34a in the exosome contents.