Molecular investigation of selected genes involved in the biosynthesis of flavonoids in two cumin ecotypes

Active substances or secondary metabolites in medicinal plants are regularly produced in response to biotic and abiotic stresses and determine the ability of plants to adapt during growth and development. In order to increase the quantity and quality of the effective substance of the green cumin medicinal plant, with the scientific name Cuminum cyminum L from the Chetrian family, we need to identify the germplasm and useful genes in domestic and wild populations of this plant. In the current research, five selected genes in the biosynthesis pathway of flavonoids in cumin, obtained from previous research based on NGS method, which had the highest expression changes in drought stress, were validated. DNA from young leaves of selected ecotypes was extracted and quality measured with agarose gel. Considering the PCR test and the observation of a single band in all cases and the lack of difference in the length of the amplified fragment among the selected ecotypes; The length polymorphism of the fragments resulting from the amplification was rejected due to the presence of deletion and addition regions between different genotypes. This experiment could not confirm the placement of the two studied ecotypes of Sadouq and Rafsanjan in terms of seed yield, essential oil and extract content, in two different groups in terms of the studied genes. Also, he could not confirm the difference in the expression of two genes (with sequence numbers DN1196 and DN32640) in drought stress and normal irrigation in the previous research