Callus induction and regeneration of C. mahaleb from leaf segment

Cerasus mahaleb L. is a member of the Rosaceae family that have been used in different industries aspharmaceutical, packaging, dyeing, chemical, sanitary, food, and perfume. For callus induction, Leaf pieces(0.5–1.0 cm2) produced by plantlets after surface sterilization in MS medium containing 2, 4-D at concentrationof 0, 0.25, 0.5, 1, 2, and 3 mg l-1 and BAP at concentration of 0, 0.5, and 1 mg l-1, placed in a growth chamberunder dark conditions. In the next step, the calli were transferred to culture medium supplemented with growthregulators of BAP, TDZ, and KIN and NAA in different concentrations for regeneration and thencharacteristics of callus induction (%), callus diameter (mm), callus type, and callus color was recorded. 100%callusing with the greatest diameter and friable and globular in texture with cream to light green in color wereobserved on MS medium at concentration of 2 mg l-1 2, 4-D in combination with 1 mg l-1 BAP. Calli showedno regeneration due to brown in color and highly soft and watery in texture.