Phytochemical analysis and anti-cancer effect of fractions and hydro-alcoholic extracts of red Algae( Gracilaria Salicornia. ) on the MCF7 and MRC5 cell line

The use of Algae was common for a long time for prevention and treatment of diseases through their variouscombinations. Red Algae (Gracilaria salicornia) has some combinations with specific chemical structures, soit can dispose singlet oxygen and absorb radicals. The objective of this study is to show the lethal effects ofGracilaria salicornia extract on breast cancer (MCF 7) and the human lung fibroblast cell line (MRC5)using the MTT method and study of compounds by GC MASS. Algae was harvested from Chabahar coast andit was prepared for hydro-alcoholic extract based on Soxhlet method and three fractions of hexane, chloroformand ethyl acetate . The five concentrations of extracts were effected and the results were evaluated. Grasilariahydro-alcoholic extract was used to assess its anti-cancer compounds and antioxidant effects by GC-Mass.Statistical analysis was performed using prism. The statistical significance of difference analyzed by one-wayANOVA/Tukey test. Result was considered significant at P< 0.05.The results showed that different concentrations of ethanolic extract and fractions significantly reduced thegrowth of MCF7 and MRC5 cell lines compared to the control group after 48 hours (P<0.05). The IC50 resultswas determined in MCF7 for total extract, fractions of ethyl acetate, chloroform and hexane it was respectivelyequal to 528, 192, 107, and 272 μg/ml and for MRC5 in order 879,205,185 and 366 μg/ml. Based on the results,the Algae can be considered as a strong chemical agent and suitable growth deterrent for this cell line.