The effect of eight weeks of cannabidiol oil consumption and swimming training on the expression of the anandamide (AEA) gene in heart tissue of rats suffering from myocardial infarction

The use of cannabidiol oil supplement can control cardiovascular risk factors. Exercise can also be effective in the rehabilitation of heart attack by strengthening the heart tissue. Therefore, the purpose of this study was to investigate the effect of eight weeks of cannabidiol oil consumption and swimming exercise on the expression of AEA gene in heart tissue of rats suffering from myocardial infarction.

The present research was experimental. 25 rats with myocardial infarction were divided into five groups of 5 series: 1) healthy control, 2) MI, 3) MI + exercise, 4) MI + CBD, and 5) MI + exercise + CBD. Myocardial ischemia was induced by subcutaneous injection of isoproterenol (85 mg/kg intravenously) in myocardial infarction rats. The swimming training program was for eight weeks. The CBD supplement was given at a dose of 50 mg/kg during eight weeks. The data of the research was analyzed by one-way ANOVA and Tukey's post hoc test (p≥0.05).

AEA in the MI group had a significant decrease compared to the healthy control group (p=0.01). Compared to the MI group, only the MI group + swimming + CBD supplement showed a significant increase in AEA (p=0.01). Compared to the MI group, only the MI+swimming+CBD supplement group showed a significant increase in AEA (p=0.01).

Eight weeks of CBD oil consumption and swimming exercise can reduce heart tissue damage caused by isopretenol -induced myocardial infarction by increasing the expression of AEA.