A Novel Design of a Non-Enzymatic Biosensor for the Detection of Hydrogen Peroxide Based on MWCNT/Co3O4/Hemoglobin Structures (Original Article)

Message:
Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Background

</span></span> Determining the concentration of hydrogen peroxide in liquids and biological samples is very important because of its effects on human health. This study aimed to design a new electrochemical biosensor based on hemoglobin to detect hydrogen peroxide in serum samples.</span></span></span></span></span></span>

Methods

</span></span> In this study, a basic science, a biosensor based on modifying the glassy carbon electrode surface with a nanocomposite consisting of cobalt oxide nanoparticles and multi-walled carbon nanotube functionalized with a carboxyl group (MWCNT/Co3</sub>O4</sub>) and hemoglobin stabilized on this nanocomposite was made as a biological recognition element.</span></span></span></span></span></span>

Results

</span></span> In optimal conditions, the biosensor was used to measure different concentrations of hydrogen peroxide. The designed biosensor showed a wide linear response range from 10 μM to 500 μM, a detection limit of 0.512 μM, and high reproducibility and stability.</span></span></span></span></span></span>

Conclusion

</span></span> In this innovative research work, MWCNTs/Co3</sub>O4</sub> nanocomposite was used to make a diagnostic biosensor. The presented biosensor showed an acceptable performance in the measurement of hydrogen peroxide in serum samples and laboratory solutions.</span></span></span></span></span></span></div>
</span></span></span></span></span>

Language:
Persian
Published:
Journal of Ardabil University of Medical Sciences, Volume:23 Issue: 89, 2024
Pages:
292 to 308
https://magiran.com/p2692260  
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