Cryopreservation and Biological Characteristics of Bangkok Chicken (Thai Game Fowl) Embryonic Fibroblast Cell Culture

Bangkok chicken, renowned for its exceptional characteristics, presents a valuable option for crossbreeding to enhance the local chicken varieties in Indonesia. Cryopreservation is widely considered a promising approach for protecting the genetic diversity of the Bangkok chicken. This research aims to investigate the biological characteristics of embryonic fibroblast cells from Bangkok chickens, with the goal of preserving the chicken strain's genetic diversity through cryopreservation methods. The fibroblast cells were derived from 8–10-day-old Bangkok chicken embryos and cultivated in DMEM media. Growth curves and Population Doubling Time were observed to assess the rate of fibroblast cell growth. Immunocytochemistry staining was employed to examine the protein expression associated with fibroblast cell adhesion. The results showed that fibroblast cells predominantly exhibited a fusiform morphology rather than an elongated cytoplasmic morphology. The cells proliferated continuously with an average population doubling time of 204 h and the maximum cell count was 7.83×104±1.7 cells/ml. The cells exhibited normal detection of protein expression, including integrin, cadherin, actin, and microtubules. It was also noted that after cryopreservation in liquid nitrogen, fibroblast cells took 4-6 days to grow and reach 80% confluency. The data collectively verifies the successful establishment of cell culture and cryopreservation for Bangkok chicken embryonic fibroblast cells.