Evaluation of Antibacterial Effect of Hydroalcoholic Pomegranate Peel Extract on Staphylococcus aureus

Staphylococcus aureus is one of the bacteria that make up the normal flora of the mouth and has been linked to conditions such as parotid infections. Parts of pomegranate were known to have antibacterial properties. Due to the properties for pomegranate and the importance of Staphylococcus aureus in the oral cavity, the incidence of dental caries and periodontal disease, finding and introducing a natural substance to replace chemical mouthwashes is crucial. in this study we investigated the antibacterial effect of pomegranate hydroalcoholic peel extract on staphylococous aureu.

For this experimental-laboratory study, Bacteria divided into 6 groups including: four groups of pomegranate peel extract with concentrations of 10, 25, 50 and 100 mg / ml, a positive control and a negative control group. Also, 3 bacterial samples were used to determine the MIC. disk diffusion test was used to measure the diameter of the growth inhibition zone. microdilution broth technique was used to evaluate the minimum concentration of bacterial growth inhibition (MIC). the obtained data were statistically analyzed by SPSS ver: 26 software. One way ANOVA test was used to examine the growth inhibition halo.

The overall results of inhibitory halo diameter evaluation showed that pomegranate extract had a inhibitory activity against Staphylococcus aureus. Bacteria were sensitive to a concentration of 25 mg / ml. That with increasing the concentration of the extract, the amount of antimicrobial effect on bacteria increases and a significant difference was shown between the diameter of the inhibition halo in four concentrations (P value <0.001) and the greatest effect was seen in 100 mg / ml concentration. In MIC study, the results showed that the effective amount of hydroalcoholic extract of pomegranate peel to prevent bacterial growth is in the range between 50 mg / ml.

According to the obtained results, the hydroalcoholic peel extract of pomegranate has antimicrobial effect and it seems that pomegranate extract can be consumed for antimicrobial use.