Cytotoxicity Comparison of Sure-seal root and Adseal Sealers on mouse fibroblast Cells:Invitro study

Biocompatibility of endodontic sealers plays an important role in the success of root canal treatment. Hence, this study was conducted to compare the cytotoxicity of bioceramic sealer ( Sure-Seal Root) and resin based sealer(Adseal), on mouse fibroblast cell line L929

In this experimental in vitro study, extraction of each sealer was obtained and incubated with dilutions of 1/2, 1/4 and 1/8 with fibroblast cell line for 48 hours in the cell culture media. MTT assay was employed to evaluate the cytotoxicity. Finally, the collected data were analyzed using One-way ANOVA and Tukey’s post-test . P<0.05 were considered statistically significant

MTT assay showed that higher dilutions of the tested materials resulted in higher viability of mouse fibroblast cell line L929. In both sealers, with increasing dilution, the survival rate of cells increased. Among two sealers, Adseal in all dilutions had lower cytotoxicity and higher cell viability percentage than sealer Sure-Seal Root ,showed that it was not significant in dilutions of 1/2and 1/8. respectively  (P=0.115) and (P=0.915), but it was significant in dilution of 1/4 .(P=0.001).

Under limitations of this invitro study ,results of this study showed that AD Seal showed less toxicity than Sure seal root in all dilutions.