Evaluation of Histomorphology and Spermatogenic Indices in Diabetic Rats Treated with Ferula assa-foetida L. Oleo Gum Resin Extract.

 For many years, medicinal plants have been used to treat many diseases, including diabetes and its side effects, due to their natural and antioxidant compounds. One of these systems that suffers from the complications of diabetes is the reproductive system. Many researches have been conducted to investigate the effectiveness of different medicinal plants on the reproductive system. In this study, the effect of Ferula assa-foetida L. gum extract on the spermatogenesis indices and testicular tissue structure of diabetic male Wistar rats was investigated.

In our study, 42 male Wistar rats were divided into 6 groups. Diabetes induced by a single dose of streptozotocin (55 mg/kg body weight). Evaluation of the effect of Ferula assa-foetida L. oleo gum extract was done by treating animals with gum extract (150 and 250 mg/kg b. w, gavage) for 42 days. After this period, the animals were anesthetized and sacrificed. After this period, the animals were anesthetized and sacrificed. Then the testicular tissue sampling was done and the samples were fixed in 10% formalin, then the preparation steps of the microscopic sections were performed included 1-dehydration, 2-clarification, 3-smearing, 5-paraffin molding, 6- Cutting with a microtome machine, 7- sticking the samples on a slide, 8- staining with hematoxylin and eosin, 9- mounting. Finally, the samples were prepared for microscopic study. The number of germinal epithelium cells of spermatogenic tubules was counted and Spermiogenesis Index (SI), Tubular Differentiation Index (TDI), Meiosis Index (MI) and Sertoli Cell Index (SCI) were measured.

 Results showed that Ferula assa-foetida L. oleo gum extract improves the tissue damages of diabetes on testicular tissue and spermatogenesis indices.

 Ferula assa-foetida L. oleo gum extract reduces testicular tissue damage caused by diabetes and has an improving effect on spermatogenesis and fertility disorders related to diabetes.