Evaluation of co-supplementation of Rutin in Simmental bull semen extender: Evaluation of kinetic parameters, sperm quality

This study investigates the effects of cryopreservation and supplementation of Simmental bull’s semen with Rutin. Therefore, this study aimed to assess motility parameters, sperm viability, oxidative stress parameters, and DNA damage to detect the optimum concentrations of Rutin for Simmental bull semen cryopreservation. Five concentrations of Rutin with 0.2, 0.4, 0.6, 0.8, and 1.0 mM were added to the freezing extender of Simmental bull sperm, respectively. The obtained results showed that the addition of 0.4 and 0.6 mM of rutin significantly improves total motility, progressive motility, plasma membrane integrity, total antioxidant capacity, reducing damage to sperm DNA, sperm viability and motility characteristics, except STR, compared to the control group. In conclusion, supplementation with Rutin in cryopreservation medium protects Simmental bull sperm against ROS attack by enhancing the antioxidative defense. Therefore, we conclude that the addition of 0.4 and 0.6 mM rutin to the Simmental bovine sperm cryopreservation extender can improve post-freezing sperm quality and may improve conception rates after artificial insemination with frozen sperm.