Metal Organic Framework Nanocomposite Adsorbents with Enzyme Function to Remove Direct Green 6 Dye Pollution

This research aims to investigate the separation of dyes by using the combination of enzyme-containing organometallic nanostructure resulting from the enzymatic layering process on the organometallic compound. For this purpose, 15 experiments were designed with the surface response method (RSM) and using the Central Composite design by Design Expert 7.0.0 Trial software and three factors of time, amount of adsorbent and solution concentration at three levels (+1 , 0, -1) was selected. In each test, a specific amount of absorbent was placed in 10 ml of 6-cation green color solution, and the amount of color removal was measured. The bioactive nanoabsorbent has a porosity of 30 Angstroms and a specific surface area of 2500 g/m2. Instrumental methods investigated the physical properties of prepared nanoparticles. The conditions of the tests included contact time (5-60 minutes), amount of absorbent (1-5 g/l), dye concentration (up to 1000 g/l) and different pH. The electron microscope image confirmed the nanostructure of the adsorbent. The maximum dye removal was determined with the amount of enzyme nanoabsorbent at five mg/L and a contact time of 45 minutes.