The effects of selenium nanoparticles on some enzymes and biochemical parameters of sperm fluid of rainbow trout (Oncorhynchus mykiss) male breeders

In this study, the effect of different levels of selenium nanoparticles was investigated on sperm fluid enzymes including aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), superoxide dismutase (SOD), catalase, lysozyme, malondialdehyde (MDA) and albumin in male breeders of rainbow trout. A number of 84 three-year-old male breeding fish were divided into four experimental groups with three replications. Fish were fed with diets containing 0 (control), 0.5, 1 and 2 mg of selenium nanoparticles per kg of diet for 60 days. The results of the analysis of the sperm fluid in different treatments did not show any significant difference in the amount of ALP, ALT and lysozyme in the sperm fluid (P>0.05). The highest amount of ALT enzyme was in the treatment of 2mg of selenium nanoparticles, and the amount of MDA, ALT and ALP of sperm fluid in other treatments was significantly higher than the control treatment (P<0.05). Based on the obtained results, in order to increase the reproductive efficiency of rainbow trout males and improve the antioxidant indices and immunity of sperm fluid, it is recommended to add 2mg of selenium nanoparticles per kg to the diet.