The effect of probiotics produced from yogurt and pure lactobacilli with and without oleic acid on blood and enzymatic parameters of healthy and diabetic male rats

In this experiment, 50 male Wistar rats were randomly divided into two groups (25 series). A group of 25 was induced with diabetes. Experimental groups included: (healthy control), healthy recipient of mixed bacteria isolated from Yogurt, healthy recipient of pure lactobacilli bacteria, healthy recipient of mixed bacteria isolated from yogurt plus oleic acid, healthy recipient of pure lactobacilli bacteria plus oleic acid, diabetic control, diabetic recipient of mixed bacteria isolated from yogurt , Diabetic receiving pure lactobacilli, diabetic receiving mixed bacteria isolated from yogurt plus oleic acid, and diabetic receiving pure lactobacilli plus oleic acid. The results showed that in healthy mice, all experimental treatments significantly increased blood glucose, cholesterol and triglycerides and decreased LDL. In healthy mice, the probiotic Lactobacillus significantly reduced the blood HDL of healthy mice. In diabetic rats, supplementation of all experimental treatments significantly reduced blood glucose, cholesterol, LDL and HDL. In this group, the greatest effect of reducing blood glucose, cholesterol and LDL was related to probiotic treatment produced from yogurt plus oleic acid. In both healthy and diabetic groups, experimental treatments significantly increased serum levels of liver enzymes (ALT, AST, ALP and CK). The greatest effect of increasing the serum activity of liver enzymes in the diabetic group was related to the treatment of lactobacillus plus oleic acid.