Design and Fabrication of Self-Assembled Nano Electrocatalyst Based on Porous Graphene/ GRP/Glucose Oxidase Enzyme for Glucose Detection and Measurement

Multilayer nanometer substrates based on graphite have emerged as a new field in the design and manufacture of biocatalysts. Layered and self-assembled Nano surfaces can provide a significant accessible surface for the immobilization of drugs, biological systems, and active enzymes and also can be used for the detection of biomarkers. In this study, a new bioelectrode was fabricated by self-assembling a porous graphene pattern with a G-rich polymer (GRP). The highly hydrophobic porous graphene was functionalized with a guanine (G) rich polymer to incorporate glucose oxidase (GOx). Graphene sheet dispersed in water with guanine (G) rich polymer. The electrochemical response of the bioelectrode to glucose was studied as a model for bioelectrocatalysis. The bioelectrode displayed a linear response to glucose concentration ranging from 0.2 to 2 mM, with a detection limit of 0.086 µM and 0.092 µA/µM/cm2 sensitivity. This study illustrates the potential of porous graphene for the construction of bioelectronics interfaces for the fabrication of electrochemical bio-devices.