Identification of miR-20a as A Potential Discerning Biomarker for Non-Invasive versus Invasive Retinoblastoma

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Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Objective
Intraocular retinoblastoma (RB) is common in kids. Although the cause of this disease is a mutation in theRB1 gene, the formed cancerous mass in different patients is seen in non-invasive states, limited to the ocular cavityor in invasive states distributed to other parts of the body. Because this tumor's aggressiveness cannot be predictedearly, these patients receive systemic chemotherapy with multiple drugs. Treating non-invasive and invasive tumorsseparately reduces chemical drug side effects. The aim of this study was to identify diagnostic biomarkers by separatingmiRNAs in blood serum from invasive and non-invasive RB patients.
Materials and Methods
In this experimental study, selected three gene expression omnibus (GEO) datasets. Twowere related to serum and tumor tissue miRNAs, and one was related to non-invasive and invasive RB gene expression.Examined RB gene-miRNA relationships. Then, we performed real-time polymerase chain reaction (PCR) on candidatemiRNAs in the Y79 cell line and patient blood samples in non-invasive and invasive retinoblastoma.
Results
Fourteen high-expression and 7 low-expression miRNAs resulted. MiR-181, miR-135a, miR-20a, miR-373,and miR-191 were common genes with differential genes between invasive and non-invasive retinoblastoma. OnlyMiR-181 was upregulated in the Y79 RB cell line. Other candidate miRNAs expressed less. Invasive retinoblastomasincreased serum miR-20a and miR-191.
Conclusion
Integrated and regular bioinformatics analyses found important miRNAs in patients’ and miR-20a, miR-191, and miR-135a can distinguish non-invasive and invasive retinoblastoma, suggesting further research.
Language:
English
Published:
Cell Journal (Yakhteh), Volume:26 Issue: 4, Apr 2024
Pages:
250 to 258
https://magiran.com/p2716825  
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