Study of Proliferation of Mesenchymal Stem cells under carboxy therapy and low-level laser

Due to the increasing importance of repairing and rejuvenating different tissues, it is necessary to pay attention to the development of treatment methods to improve treatment results. Low-level lasers are currently used to repair skin tissues. The carboxytherapy method has become particularly important due to its non-invasive treatment. In this method, some carbon dioxide is injected into the tissue.
It is produced naturally by the cells of the body, but when the amount of CO2 becomes too much, it forces the body to respond. When carbon dioxide enters the body, the circulatory system in the treated area is stimulated. And then tries to control the condition using blood vessels. Due to the clinical effects of low-level laser and also the carboxytherapy method, it is necessary to study carboxy effects and simultaneously the low-level laser at the cellular level.
In this study, the proliferation and viability of Adipose Mesenchymal Stem cells (MSC) have been studied under Laser and carboxy therapy treatments to help develop more effective protocols.

Adipose Mesenchymal Stem cells were cultured in DMEM F12 + 10% fetal bovine serum (FBS), 100 U/ml penicillin and 100 mg/ml streptomycin. The cells were placed in an incubator with 5% CO2 humidified atmosphere at 37°C. MSC stem cells were irradiated by low-level laser with various doses of 2,3,5 and 7 J/cm2 and also cells were exposed to carboxy with different concentrations (with speeds and Different volumes) by Carboxy Therapy device. viability of cells were done by MTT test.

The results of this study illustrated the viability and proliferation of cells under the laser and carboxy therapy increased and at the simultaneous laser and carboxy treatment showed further increase than the control group.

Based on the findings of this study, the use of carboxytherapy as well as the simultaneous use of laser with an optimal dose can be used as an alternative method in skin repair.