Effect of probiotic supplementation with aerobic training on hepatic IL-6 gene expression in non-alcoholic fatty liver rats

non-alcoholic fatty liver disease NAFLD is the most common liver disease in the world, the most important cause of non-alcoholic fatty liver disease is poor eating habits and sedentary lifestyles that cause obesity. The aim of this study was the effect of aerobic training and probiotic consumption on gene expression of IL6 liver tissue in rats with non-alcoholic fatty liver.

In this experimental study, 32 male Wistar rats, 200-250 gr, were randomly divided into 4 groups: healthy group, modeled group (steatosis), steatosis + probiotic group, steatosis + probiotic + training group. To create a fatty liver model, tetracycline at a dose of 100 mg / kg at a volume of 1.5 cc per rat was gavaged daily for two weeks. The main training program was for eight weeks/3times per week. In the first week with a speed of 18 meters per minute, the time started 10 minutes and every week quickly, 1-2 meters per minute and time was added to 10 minutes so that in the fourth week the speed to 22 meters per minute and time to 40 minute has arrived. Probiotic groups received 109 CFU / ml of Lactobacillus ramensus by gavage daily for 8 weeks and 5 days a week. 48 hours after the last training session, 10 ml of blood was taken directly from the hearts of rat to test for total cholesterol (TC) and liver tissue samples were taken to test for IL6 gene expression. One-way analysis of variance and Tukey post hoc test was used P≤0.05.

There is a significant difference between the IL-6 gene variable in the groups (P=0/000), Induction of fatty liver in rats was associated with a significant increase in interleukin-6 gene levels, these changes were significantly reduced after taking probiotics (P=0/000), Also probiotic consumption with aerobic training had a significant reduction compared to other groups. However taking probiotic with aerobic training had no significant effect on TC of rats with non-alcoholic fatty liver (P=0/306).

Probably, performing aerobic training along with taking probiotic can have a better effect in controlling non-alcoholic fatty liver disease.