Prediction of the best signal peptide for periplasmic expression of melittin peptide in Escherichia coli
Periplasmic expression of antimicrobial peptides is one of the most important issues in cloning and protein expression systems. In the present study, bioinformatics methods were used to predict the best signal peptides for periplasmic expression of the melittin peptide in Escherichia coli. Therefore, the sequence of 12 signal peptides was prepared from signal peptide databases. In order to choose the best signal peptide, periplasmic expression, sub-cellular localization site, solubility, physical and chemical properties of signal peptides were analyzed by Signalp6, Psort, Protein-sol, PRED-TAT, and Portparam servers. Six of the 12 signal peptides passed the Signalp6 filter, and only two passed Psort. After examining the solubility of signal peptides and investigating their physical and chemical properties, it was determined that zraP has the most favorable characteristics. Finally, zraP could be considered the best signal peptide for the melittin expression. Our results can be used for periplasmic expression of melittin in Escherichia coli host.
- حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران میشود.
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