The Effect of Resistance Training and Nanocurcumin on Tumor Tissue Inflammatory Markers (NF-κB and IL-1α) in Rats with Glioblastoma Multiforme

The aim of this study was to investigate the effect of resistance training and nanocurcumin supplementation on protein amounts of NF-κB and IL-1α involved in tumor metabolism in rats with glioblastoma multiforme (GBM).

30 male Wistar rats were divided into 5 groups namely, healthy control, GBM, GBM+resistance training (RT), GBM+nanocurcumin supplement (NCUR), and GBM+RT+NCUR. GBM was injected into the frontal cortex of rats. The training group performed incremental resistance training on the ladder for 4 weeks, 3 days per week. At the end, the rats were sacrificed and the histological changes of the brain tumor were evaluated by H&E method, and also the expression of NF-κB and IL-1α protein were measured by western blot method.

Compared to the healthy control group, the GBM group showed significant tissue changes (increasing tumor area) and increased IL-1α protein levels (p≤0/05). In the analysis of tissue changes, it was found that the GBM+RT, GBM+NCUR and GBM+RT+NCUR (p≤0/05) groups showed a significant reduction in the brain tumor area compared to the GBM group. Also, the GBM+RT+NCUR group showed a significant decrease in tumor area compared to the GBM+RT and GBM+NCUR groups (p≤0/05).

It seems that, to evaluate the changes of inflammatory markers involved in tumor metabolism, longer treatment duration, different dosage of supplements, different exercise training, intensity and volume of exercise training should be used. Also, the progress of the disease can be one of the factors affecting the non-change of the molecular factors of the present study.