Preservation of Buck Semen Quality During Chilling Storage Using Coenzyme Q10

Storing spermatozoa in a cold environment decreases spermatozoa metabolism while maintaining sperm fertility. Because of their unique physiological characteristics, sperm cells’ reproductive ability in small ruminants is reduced by cooling. This study aimed to assess the impact of supplementing chilling medium with coenzyme Q10 (CoQ10) on the quality parameters of buck’s semen after chilling at 4°C. Semen was collected, diluted, and divided into five groups with different CoQ10 supplementation concentrations (0, 1, 2, 5, and 10 µM CoQ10). Collected semen were stored at 4°C for 50 h. The motility, mitochondrial activity, membrane integrity, viability, and lipid peroxidation were measured after 0, 25, and 50 h of chilling. According to the findings, different concentrations of CoQ10 had no impact (P>0.05) on semen at time 0 of storage. After 25 and 50 h of storage, supplementing the chilling medium with 5 µM CoQ10 increased (P≤0.05) progressive motility (PM), total motility (TM), viability, mitochondrial activity, and membrane integrity compared to other groups. Furthermore, 5 µM CoQ10 produced less lipid peroxidation (P≤0.05) after 25 and 50 h than the other groups. Adding 5 µM CoQ10 to the buck’s semen cooling medium is an effective procedure for protecting the quality of buck spermatozoa during cooling storage.