EVALUATION OF THE RELATIONSHIP BETWEEN −159 C>T CD14 POLYMORPHISM WITH PEPTIC ULCER DISEASE AND HELICOBACTER PYLORI INFECTION IN GUILAN PROVINCE POPULATION, NORTHERN IRAN

Peptic ulcer disease causes open sores in the lining of the stomach or duodenum. Helicobacter pylori infection is the main cause of gastrointestinal ulcers. CD14 is a pattern recognition receptor in the cell membrane that identifies bacterial products and plays a key role in the early stages of immune and inflammatory responses. The aim of this study was to investigate the association between the C159T polymorphism of the CD14 gene in patients with peptic ulcer and Helicobacter pylori infection.

In this case-control study, 137 patients with peptic ulcers and 123 healthy individuals were evaluated. Genomic DNA was extracted from gastric biopsies, and genotypic frequency in patients and the control group was determined using the Tetra primer ARMS-PCR method. Data analysis was performed using SPSS v.26 software.

The frequency of CC, CT, and TT genotypes in patients was 24.8%, 41.6%, and 33.5%, respectively, while in healthy individuals, it was 8.9%, 43.1%, and 47.3%, respectively. Statistical analysis revealed a significant relationship between the C159T polymorphism of the CD14 gene and peptic ulcer disease and Helicobacter pylori infection (P = 0.0003).

The results of the statistical analysis showed that the presence of the T allele at the C159T position of the CD14 gene is considered a risk factor for peptic ulcer disease and Helicobacter pylori infection. However, further studies on other CD14 gene polymorphisms and larger populations are needed.