Effects of eight-week of HIIT along with green tea on serum of acetylcholinesterase, amyloid beta-42 and malondialdehyde in untrained obese elderly men

The use of medicinal plants along with exercise especially in obese people has received much attention due to the modulation of inflammatory and oxidative responses. Therefore, the aim of this study was to investigate the effect of eight weeks of high intensity interval training (HIIT) and green tea supplement on serum levels of acetylcholinesterase (AChE), amyloid beta-42 (Aβ42) and malondialdehyde (MDA) in untrained obese elderly men.

Forty eight obese elderly men (age 65.42±3.16 years and body mass index 30.93±1.15 kg/m2) were randomly divided into four groups of 12 people including HIIT, supplement green tea, HIIT+ green tea supplement and control- placebo. HIIT training was carried out for eight weeks and three sessions per week. Green tea supplement in the form of six capsules per day in the amount of 450 mg was received by the target groups. Testing was done 48 hours before and after the last training session. The AChE and AB42 were measured by ELISA and MDA by TBARS method. Results were extracted using by analysis of variance with repeated measurement, analysis of variance two -way and Tukey’s post hoc tests at a significance level of p<0.05.

The most significant changes (decrease) in body mass index (p=0.01, 3.50%) and percent body fat (p=0.0001, 11.07%); and a significant increase in maximum oxygen consumption (p=0.02, 9.22%) was observed in the HIIT+ green tea group. The combined effect of HIIT and green tea supplementation showed a significant decrease in AChE (p=0.0001, 19.91%), Aβ42 (p=0.0001, 27.99%), and MDA (p=0.01, 26.09%). Moreover, the HIIT caused significant decrease  in AChE (p=0.02, 15.67%), Aβ42 (p=0.01, 16.22%) and MDA (p=0.01, 14.14%); and green tea supplement caused a significant decrease in Aβ42 (p=0.023, 6.19%).

The combined intervention of HIIT with green tea supplement compared to the effect of each one alone, causes a further improvement of inflammatory and oxidative indices in obese elderly men.