Expression of Nitric Oxide Synthase(NOS) during Embryonic Development of the Rat Optic Vesicle
Author(s):
Abstract:
NO is a free radical that regulates a variety of developmental modulation processes. NO is synthesized by NOS and it acts as a neurotransmitter, neuromodulator or second messenger molecule in the central nervous system. Since NO production may be different before or after birth, the expression of neuronal nitric oxide synthase was examined and analysized during the development of rat optic vesicle from embryonic day E14 to E18 by histochemical procedures. The samples were frozen and cut on a cryostat and then studied by using the light microscope. Expression of nNOS was first seen on E14 in cells of Cajal-Retzius located in the marginal zone of optic vesicle. NADPH-d persisted in this layer throughout the embryonic period and began to decrease on E20. On E16, the optic vesicle had four NADPH-d positive layers. On E18,NADPH-d reactivity observed at low magnification was clearly defined in the fifth layer. In the late stages, the most notable feature was a decrease in histochemical reaction of the marginal zone, i.e. the fourth layer showed less staining than the rest of the cortical plate. The observations suggested that nitric oxide is synthesized during embryonic life processes and it changes with ageing in the rates of NOS-reactive cells in embryonic life and maturation processes. These results indicate that NOS makes different contributions in the optic vesicle NO production along with ageing.
Keywords:
Language:
Persian
Published:
Razi Journal of Medical Sciences, Volume:12 Issue: 2, 2005
Page:
393
https://magiran.com/p304638
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یکساله به مبلغ 1,390,000ريال میتوانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
- حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران میشود.
- پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانههای چاپی و دیجیتال را به کاربر نمیدهد.
In order to view content subscription is required
Personal subscription
Subscribe magiran.com for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!