Detection of Human Papilloma Virus DNA Genotypes in oral squamous cell carcinoma

Squamous Cell Carcinoma (SCC) is the most prevalent cancer of oral cavity which many etiologic factors have been identified in the development of it. Recently, Human Papilloma Virus (HPV) has been studied as one of causative factor of oral SCC. The aim of this study was detection of relationship between HPV and oral SCC by Polymerase Chain Reaction (PCR) and comparing with normal oral mucosa. Methods & Materials: In this study 51 oral SCC and 28 controls from normal oral mucosa which all of them were as paraffin block were evaluated genetically by PCR.HPV positive samples were tested for HPV 6,11,16,18,31,33 type.

HPV DNA was detected in 21.5% (11.51) of the cases and 3.6% (1.28) of the controls with significant difference between two group (P<0.05). HPV positive cases then were examined for typing PCR.HPV 6 was more prevalent with 4 cases and HPV 31,6,33,and 35 were identified in 3, 2, 1, 1 cases respectively. No positive cases with HPV 6 and 11 was found. There was no significant difference between male and female HPV positive cases.4 HPV positive cases of oral SCC were located in the floor of mouth.4 HPV positive cases used from chewing tobacco.

It seems that HPV has an etiologic role in the development of the oral SCC, however, it needs more investigations.