Typing of HLA Class I by Polymerase Chain Reaction-Sequence Specific Oigonucleotide Primer

HLA compatibility between transplant donor and recipient is one of themajor determinants of transplant outcome. To determine HLA class I by PCR- Sequence-Specific Oligonucleotide Probe (PCR-SSOP) in cord blood donors. Genomic DNA of 142 cord blood samples registered at the Cord Blood Bank of Iran at Hematology، Oncology، and Bone Marrow Transplantation Research Center، was prepared and HLA class I was determined by the PCR-SSOP. A total of 284 HLA-A alleles was identified of which A*02 and A*24 were the most common. Among 284 HLA-B and HLA-C alleles، B*35، B*51، Cw*4 and Cw*12 were the most frequent alleles in the studied population. Amplification of HLA loci with PCR-SSOP has proved to be a reliable method for HLA-A، -B and -C genotyping.