Possibility of HAV diagnosis in milk by ELISA technique

Food borne viral infections are recognized increasingly as human illneses. Hepatitis A virus (HAV) is an important pathogen which has been responsible for water and many food – borne outbreeks especially milk. In this study for approaching to best diagnosis method of HAV in milk by ELISA technique, various dilutions of HAV antigen (1 ml/L, 10-3 ml/L, 10-6 ml/L, 10-9 ml/L) were added to UHT milk. Various treatments for virus(antigen) isolation, showed that milk with acidic coagulation, following filtration with paper and membrane filters, had the most optical density. It`s indicated that this treatment resulted the best diagnosis. Also it has been revealed that this method has not any sensitivity for quantitative determination of HAV(antigen) in milk.