Sprague Dawley The Effect of Lipopolysaccharide(LPS) on Nitric Oxide(NO) and Glucose Concentration in the Serum of SD Rats(Sprague Dawley

Nitric Oxide(NO) is one of the ten smallest lipophilic molecules synthesized from L-Arginine, O2 and NADPH by nitric oxide synthase enzyme(NOS). NO has various biological activities in different systems. For instance, it plays both protective and cytotoxic roles in immune system, while in cardiovascular system it has physiologic role which controls vascular tonicity. One of the NOS isoforms known as iNOS(inducible NOS) can be induced by variety of some gram negative bacteria membrane that leads to NO production for long period of time. Due to important biological roles of glucose and NO, the present study was designed to investigate the effect of LPS on the level of glucose and NO in serum of SD rats. In this study 50 SD male rats with the average weight of 250-300 gram were chosen. Rats were divided into five groups(10 rats in each group). First group received 0.2mg/kg, second group 0.4mg/kg, third group 0.8 mg/kg LPS, fourth group 0.8ml/kg salin via IP injection and the fifth group did not receive any compound. After blood collection and separation of serum, NO level was measured by Griess reagents and glucose by colorimetric method. The obtained results showed that with increased LPS injection, the level of NO increased in group 1, 2 and 3 respectively. The latter group had the maximum level of NO as compared to control group(P<0.05). Glucose concentration increased significantly in third group(P<0.05). It is concluded that increased level of NO production was due to induction of iNOS enzyme by LPS and was dose dependent, and increased level of NO led to increased level of glucose concentration.