The relationship between MSX1 gene mutation and tooth agenesis with molecular approach RLFP-PCR

The important role of genetics has been recognized in recent years with respect to the understanding of tooth agenesis (TA). Identification of genetic mutations in individuals with tooth agenesis or other dental anomalies will enable preclinical diagnosis and improves orthodontic treatment. The aim of the present study is to assess the relationship between T.A and MSX1 mutation. Methods & Materials: Case group consisted of 20 individuals with T.A and control group consisted of 20 individuals with complete set of dentition. Blood samples were collected and DNA’s were extracted with P.C.I Ext Method. DNA’s were amplified with PCR technique and after addition of Ban II on PCR product, presence or absence of MSX1 mutation was evaluated. Evaluation was based on presence of additional DNA band in gel of electrophoresis in case group. Chi square test was used to compare two qualitative traits. Ban II had no effect on DNA of control group (195 bp) and digested the defected Allele in case group (106 bp and 89 bp.) (P-valve <0/001). In control group no mutation had occurred and all of individuals in case group, had heterozygous mutations and there is a high significant statistical relation ship between T.A and MSX1 mutations. Given the absence of tooth follicle in dental radiography, formation or agenesis of the mentioned tooth can be determined by means of explained method of present study. After all, performing a blood test can prognosticate whether the future tooth can be developed or not.