Anti inflammatory and analgesic activity of Glaucium grandiflorum extract

Background and Various species of the plant grandiflorum have different applications in Ïran traditional medicine, such as in treatment of dermatitis. Çonsidering the therapeutic property of papuveraceae, this study was designed in order to determine the anti inflammatory and antinociceptive property of Glaucium grandiflorum. Ëxtraction was done with methanol by percolation method. Ânti inflammatory property of the extract was determined by carrageenan test for the doses of 4,10,20, 40, 80, 160 and 200 mg/kg of body weight as compare to indometacin for the doses of 2. 4, 6, 8 and 90 mg/kg of body weight. Ântinociceptive property of the extract with formalin and hot plate was studied for the doses of 60,70,80 and 90 mg/kg of body weight as compare to morphine for the doses of 5 and 10 mg/kg of body weight. The integrity of motor coordination was assessed with a rotarod apparatus. Âcute toxicity (LD50) of the extract was determined by probit method. Data were analyzed by variance test and Newman- keuls test with P<0.05. 200 mg/kg dose of the extract had more anti inflammatory effect as compare to indomethacin at the doses of 10 mg/kg (P<0.01) and 8 mg/kg (P<0.001). Âll the used doses had more antinociceptive effect as compare to the control group, (P<0.001). Ântinociceptive effects of 80 and 90 mg/kg of body weight of the extract in the first phase was more than morphine for the dose of 5 mg/kg body weight. Ïn the second phase, the antinociceptive effect of 70, 80 and 90 mg/kg of body weight was more than morphine for the dose of 5 mg/kg of body weight P<0.001. There was no significant difference in the doses of 80 and 90 mg/kg of body weight. Âdministration of the extract with the doses of 60, 70,80 and 90 mg/kg of body weight, raised the pain threshold at 45 minutes of observation, as compare to the control group. Ëxtract fluid of this plant had no effect on motor coordination of the animals. Âcute toxicity of the extract was determined to be about 797.94 mg/kg administered intraperitonealy.Çonclusion: The present study suggets that this plant has anti inflammatory and antinociceptive effects.