Construction of an expression vector containing HSV-l glycoptotein B(gB) gene to express in mammalian cell lines

Abstract Glycoprotein B of herpes simplex virus type 1 (gB-l) is one of the surface membrane glycoproteins of the virus, which is of great importance in virus infectivity, and is a target for immune system. The main aim of th is research was to express gB-I gene in eukaryotic cells. The gB-I gene was subcloned from pAc-gB plasmid to pcDNA3 expression vector under control ofCMV promoter. The right orientation of the inserted gene was then determined using restriction enzyme analysis. In order to examine expression of the gene in eukaryotic cell, the plasmid was transfected to the COS-7 cells, using liposome. In vitro expression of gB protein was determined using immunoflurescent techniques Results showed that the protein of interest had been synthesized in the cells, 72 hours after transfection. Expression of gB in COS-7 cells and its interaction with specific antibody, confirmed efficacy of constructed vector in expression of gB protein with suitable antigenic structure that can be used in vaccination studies.