Construction a DNA Vaccine Containing Human Papillomavirus Type 16 Early Genes as a Potential Vaccine for Cervical Cancer Prevention and Therapy

Some of the human papillomaviruses (HPVs) can infect genitaltracts and are sometimes associated with anogenital tract cancers. HPVs induced cervical cancersthrough the expression of E6 and E7 genes by inactivating the tumor suppressor proteins. In thisstudy, E6 and E7 genes were chosen in order to construct an expression vector which is able toexpress target proteins.Patients and This experimental investigation was performed in Virology Departmentof Tarbiat Modares University. An expression vector containing human papillomavirus type 16 E6and E7 genes was constructed. The accuracy of the plasmid was confirmed by polymerase chainreaction (PCR) and restriction enzyme analysis. The construct was transfected into the eukaryoticcells and its ability for protein production was confirmed by Western blotting. The colonies containing desired plasmid have the fragment about 995 bp. For confirmingthe ability of the construct for protein production in eukaryotic cells, Western blotting was doneusing the lyses-cells as antigen and they showed the desired bands using monoclonal antibodies. The designed vector can consider as a based vaccine for construction a therapeuticvaccine in suitable vectors for gene therapy in order to administration in Iranian patients withcervical cancer.