Study of Fibroblast Cells Behavior on the Acrylic Acid Co2 Pulsed Laser Grafted Silicone Elastomer for Tissue Engineering Application

In this study Silicone as a base material was selected and poly (acrylic acid) grafted on it using a CO2 pulsed-laser. The effect of laser parameters on weight decreases of silicone sheets were determind by weighing of samples before and after irradiation. In addition the effect of laser pulse density on the samples surfaces were investigated by characterization with Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR), Scanning Electron Microscopy (SEM), water drop contact angle and water surface uptake measurements.Then, L929 fibroblast cells were cultured on the samples in order to determine cell adhesion, spreading and proliferation. Results obtained from in vitro experiments after 12 and 72 hours showed considerable cell adhesion, spreading and proliferation on, silicone surfaces whereas poly (acrylic acid) grafted silicone surfaces showed poor adhesion spreading, and proliferation on basis of the amount of grafted poly (acrylic acid).These results show that the modified surfaces control cell adhesion, spreading and proliferation, while they can be used for immobliziation of proteins and polysaccharides for tissue engineering.