Somatic embryogenesis and plant regeneration in Canola (Brassica napus L.)

In this study a simple method for the induction and development of somatic embryos from Canola (Brassica napus L.) hypocotyl explants is determined and reported. Hypocotyl explants were found to be more suitable than other plant parts for somatic embryogenesis. A low concentration of sucrose (20 g/lit) enhanced somatic embryogenesis, but greater concentrations (60 g/lit) decreased embryogenetic potential of the Calli. On modified MS medium containing 2% sucrose, 1 mg/lit 2,4-D, 2 mg/lit NAA and 2 mg/lit BAP each explant developed calli. Different NaCl concentrations (0, 2, 3, 4, 5, 6, 7, and 8 mg/lit) were tested and somatic embryogenesis was improved in the presence of 6 mg/lit NaCl. Embryoids developed shoots on the same medium and rooted after being transported to a medium supplemented with 1 mg/lit IBA. Regenerated plants were transfered to pots after adaptation to dry condition and produced flowers normally.