Identification of Candida species associated with vulvovaginal candidiasis by Multiplex PCR method

Author(s):

Mahmoudi Rad M. , Zafarghandi As , Amel Zabihi M. , Mirdamadi Y. , Rahbarian N. , Abbasabadi B. , Shivaei M. , Amiri Z

Message:
Abstract:
Vulvovaginal candidiasis is a fungal disease with itching, and vaginal thick white discharge. Most of non-albicans species have less sensitivity to azoles. So, definition of candida species which lead to vulvovaginal candidiasis is very important to perfect usage of drugs. In the present study 191 Candida isolates from 175 patients who admitted in Gynecology department of Mahdieh Hospital during the period 1385-1387 were identified by multiplex PCR. One hundred seventy five vaginal swab specimens from patients were cultured on Sabouraud Dextrose Agar (SDA). The internal transcribed spacer 1 (ITS1) region between the 18S and 5.8S rRNA genes and a specific DNA fragment within the ITS2 region of
Language:
Persian
Published:
Tehran University Medical Journal, Volume:67 Issue: 9, 2009
Page:
623
https://magiran.com/p685231