Effect of phytohormone and explant on callugenesis and regeneration of sainfoin (Onobrychis sativa L.)

To study the effects of phytohormone and explant on the callugenesis and regeneration of Sainfoin (Onobrychis sativa L. var. Chadegan) an experiment was conducted in the bitechonolgy laboratory of Sciences and Research Unit,Islamic Azad University, Tehran, Iran in summer and spring of 2006. In this experiment, two phytohormones (2,4-D and BAP(and 4 explants (cotyledon leaf, apical meristem, stem, and root) were used. The highest number of callus was obtained on the MS medium containing 2,4-D (4 mg.l-1) + BAP (1.5 mg.l-1) + apical meristem explant and 2,4-D (2 mg.l-1) + BAP (1 mg.l-1) + apical meristem explant with means of 1.77 and 1.76 callus, respectively. The highest regeneration was obtained in MS medium containing BAP (4 mg.l-1) with mean of 1.6 regenerated plants. A new method for in vitro micropropagation of Sainfoin (Onobrychis sativa Var Chadegan) was developed. Seedlings with root were transferred to greenhouse and continued growing.